Papanicolaou's solution 3b polychromatic solution EA 50 used in nuclear staining, for cytology, Sigma-Aldrich®

Supplier: MilliporeSigma
Danger

1.09272.0500 1.09272.2500 1.09272.1000
EM1.09272.1000EA 199.17 CAD
EM1.09272.1000 EM1.09272.0500 EM1.09272.2500
Papanicolaou's solution 3b polychromatic solution EA 50 used in nuclear staining, for cytology, Sigma-Aldrich®
Papanicolaou's solution 3b polychromatic solution EA 50

Used to demonstrate the differentiation of squamous cells in gynecological cytology


The cytological staining technique developed by Papanicolaou is still the standard method in cancer and hormone cycle diagnosis. Four different polychrome solutions are available; they differ in the various concentrations of the components and produce different cytoplasm stains. In the last staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown. The polychrome solution is used to demonstrate the differentiation of squamous cells. In gynecological cytology Polychrome solution EA 31 (3a) and EA 50 (3b) are mostly used. In non-gynecological cytology Polychrome solution EA 65 (3c) and EA 65 (3d) are used. It is an IVD product and CE certified.

Density: 0.82 g/cm³ (20 °C)
Flash Pt: 17 °C
Storage Temperature: Ambient
UN: 1987
ADR: 3,II
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Specification Test Results

Suitability for microscopy (Vaginal smear) passes test
Nuclei blue to dark violet
Cyanophilic cytoplasm blue-green
Eosinophilic cytoplasm pink

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