Papanicolaou's solution 3b polychromatic solution EA 50 used in nuclear staining, for cytology, Sigma-Aldrich®
Supplier: MilliporeSigma
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Danger
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Used to demonstrate the differentiation of squamous cells in gynecological cytology
The cytological staining technique developed by Papanicolaou is still the standard method in cancer and hormone cycle diagnosis. Four different polychrome solutions are available; they differ in the various concentrations of the components and produce different cytoplasm stains. In the last staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown. The polychrome solution is used to demonstrate the differentiation of squamous cells. In gynecological cytology Polychrome solution EA 31 (3a) and EA 50 (3b) are mostly used. In non-gynecological cytology Polychrome solution EA 65 (3c) and EA 65 (3d) are used. It is an IVD product and CE certified.
Density:
0.82 g/cm³ (20 °C) Flash Pt: 17 °C Storage Temperature: Ambient |
UN:
1987 ADR: 3,II |
Specification Test Results
Suitability for microscopy (Vaginal smear) | passes test |
Nuclei | blue to dark violet |
Cyanophilic cytoplasm | blue-green |
Eosinophilic cytoplasm | pink |
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