Agarose II, powder, low melting for biotechnology
Supplier: G-Biosciences
G-Biosciences offers several different biotechnology-grade agarose powders. Agarose II has a low melting and gelling temperature compared to standard agaroses. The low melting temperature allows for the recovery of undamaged nucleic acids below the denaturation temperature. The low gelling temperature ensures that the agarose will be in a liquid state at a temperature range where in-gel manipulations can be performed without prior extraction from the agarose slice.
- Lower gel strength compared to standard agarose
- Higher clarity
- Easy to handle
Applications:
Electrophoresis of DNA Fragments >1000bp
Tissue and Cell Culture
Viral Plaque Assays
Storage Temperature:
Ambient |
MDL Number:
MFCD00081294 CAS Number: 9012-36-6 EINECS: 232-731-8 |
Specification Test Results
Moisture | ≤ 7% |
Ash | ≤ 0.4% |
EEO (electroendosmosis) | ≤ 0.12 |
Sulfate | ≤ 0.12% |
Clarity 1.5% (NTU) | ≤ 4 |
Gel Strength 1.5% (g/cm2) | ≥ 500 |
Gelling Temperature 1.5% (ºC) | 24-28 |
Melting Temperature 1.5% (ºC) | ≤ 65.5 |
DNase/ RNase Activity | None Detected |
DNA Resolution ≥1000bp | Finely resolved |
Gel background | Very low |
Appropriate Agarose Concentrations - 1X TAE BUFFER | |
0.75 % | 500-20,000bp |
1.00 % | 300-16,000bp |
1.25 % | 250-10,000bp |
1.50 % | 200-5,000bp |
1.75 % | 100-2,500bp |
2.0 % | 50-1,500bp |
Appropriate Agarose Concentrations - 1X TBE BUFFER | |
0.75 % | 500-12,000bp |
1.00 % | 300-8,000bp |
1.25 % | 200-4,000bp |
1.50 % | 150-3,000bp |
1.75 % | 100-2,000bp |
2.0 % | 50-1,000bp |
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