BsaI-HF®v2, Restriction Enzymes, New England Biolabs

Supplier: New England Biolabs (NEB)

BsaI-HF®v2, Restriction Enzymes, New England Biolabs

BsaI-HF®v2, Restriction Enzymes, New England Biolabs

R3733L R3733S

CA76284-692EA 480 CAD

CA76284-692 CA76284-694

BsaI-HF®v2, Restriction Enzymes, New England Biolabs

Nucleic Acid Reagents Restriction Enzymes

High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (CutSmart® Buffer).

Maintains high activity levels in T4 DNA ligase buffer
100% activity in CutSmart Buffer
Time-Saver™ qualified for digestion in 5 to 15 minutes

Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence.

BsaI-HFv2 has been optimized for Golden Gate Assembly. BsaI-HFv2 also works well for any protocol requiring DNA cutting by BsaI. This is the recommended enzyme for any purpose requiring digestion at the recognition sequence 5'-GGTCTC(N1)/(N5)-3'.

This enzyme is produced from E. coli strain that carries the cloned and modified BsaI gene from Bacillus stearothermophilus 6-55.

Ordering information: Concentration: 20000 U/ml

Delivery information: Supplied with gel loading dye, purple (6X) and CutSmart® buffer (10X).

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