Human Relaxin 3 AccuSignal ELISA Kit
- Natural and recombinant human Relaxin 3.
- There is no detectable cross-reactivity with other relevant proteins.
- Expression system for standard: E.coli; Immunogen sequence: D119-C142(A)&A27-R53(B)
Relaxin-3 is a neuropeptide which is highly conserved in species ranging from flies, fish, rodents and humans. It is a member and ancestral gene of the relaxin family of peptides, which includes the namesake hormone relaxin (designated 'H2 relaxin' in humans) which mediates peripheral actions during pregnancy and which was found to relax the pelvic ligament in guinea pigs almost a century ago. The broad distribution of relaxin-3 fibres/RXFP3 within several key neuronal circuits suggests an ability to modulate a broad range of behaviours. This has been confirmed in a growing number of rodent studies, which demonstrate relaxin-3 is able to modulate arousal, the response to stress, feeding/metabolism and memory; and likely plays a role in the generation/regulation of hippocampal theta rhythm.
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human Relaxin 3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma( heparin) to each empty well. It is recommended that each human Relaxin 3 standard solution and each sample be measured in duplicate.
