Human KLK12 ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemicals

KOA0670

76236-968EA 1150.24 CAD

76236-968

Human KLK12 ELISA Kit, Rockland Immunochemicals

Assays ELISAs

Human KLK12 AccuSignal ELISA Kit

Natural and recombinant human KLK12.
There is no detectable cross-reactivity with other relevant proteins.
Expression system for standard: NSO; Immunogen sequence: A18-N248

Kallikrein-12 is a protein that in humans is encoded by the KLK12 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord. The expression of KLK12 is downregulated at the mRNA level in breast cancer tissues and is upregulated by steroid hormones in breast and prostate cancer cell lines.

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10,000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human KLK12 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KLK12 standard solution and each sample be measured in duplicate.

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