Human MMP-12 ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemical

KOA0581

76235-702EA 1041.22 CAD

76235-702

Human MMP-12 ELISA Kit, Rockland Immunochemicals

Assays ELISAs

Human MMP-12 AccuSignal ELISA Kit

Natural and recombinant human MMP-12.
There is no detectable cross-reactivity with other relevant proteins.
Expression system for standard: NSO; Immunogen sequence: L17-C470

Matrix metalloproteinase-12(MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human MMP-12 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human MMP-12 standard solution and each sample be measured in duplicate.

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