Human IL-18 ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemicals

KOA0523

76236-934EA 1150.24 CAD

76236-934

Human IL-18 ELISA Kit, Rockland Immunochemicals

Assays ELISAs

Human Interleukin-18 AccuSignal ELISA Kit

Natural and recombinant human IL-18.
There is no detectable cross-reactivity with other relevant proteins.
Expression system for standard: E.coli; Immunogen sequence: Y33-D197

Interleukin(IL)-18, also called Interferon-gamma-inducing factor(IGIF), augments natural killer(NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1(Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human IL-18 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IL-18 standard solution and each sample be measured in duplicate.

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