Human IL-27 ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemicals

KOA0472

76237-328EA 1150.24 CAD

76237-328

Human IL-27 ELISA Kit, Rockland Immunochemicals

Assays ELISAs

Human Interleukin-27 AccuSignal ELISA Kit

Natural and recombinant human IL-27.
There is no detectable cross-reactivity with other relevant proteins.
Expression system for standard: NSO; Immunogen sequence: EBI3(R21-K229)+P28(F29-P243)

Interleukin-27(IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits; Epstein-Barr virus(EBV)-induced gene 3(EBI3)(also known as IL-27B) and IL27-p28(known as IL-30). IL-27 is produced by antigen-presenting cells.IL-27 plays an important function in regulating the activity of B- and T-lymphocytes. IL-27, a potent inhibitor of T(H)-17 cell development, may be a useful target for treating inflammatory diseases mediated by these cells.

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human IL-27 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IL-27 standard solution and each sample be measured in duplicate.

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