Human Gp130 - IL6ST ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemical

KOA0185

76237-574EA 1150.24 CAD

76237-574

Human Gp130 - IL6ST ELISA Kit, Rockland Immunochemicals

Assays ELISAs

Human Gp130 - IL6ST AccuSignal ELISA Kit

Natural and recombinant human Sgp130.
There is no detectable cross-reactivity with other relevant proteins.
Expression system for standard: sf21; Immunogen sequence: L24-E619

GP130(Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-bound gp130. Gp130 is an important part of many different types of signaling complexes. Gp130 molecules with mutations in either of these segments could not transduce a growth signal.

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml human GP130 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human GP130 standard solution and each sample be measured in duplicate.

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