Monarch® Total RNA Miniprep Kit, New England Biolabs

Supplier: New England Biolabs
Monarch®
T2010S T2010S
CA103529-148EA 407 CAD
103529-148 CA103529-148
Monarch® Total RNA Miniprep Kit, New England Biolabs
Nucleic Acid Reagents Nucleic Acid Purification Kits and Reagents
Quickly and easily purify up to 100 µg of high quality total RNA from multiple sample types – all with one kit.

  • Activity is not inhibited by an α1-3 Fucose residue on the chitobiose core
  • Effectively purifies total RNA of all sizes, including small RNAs >20 nt
  • Efficient genomic DNA removal (column and DNase I-based)
  • Excellent value

The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues.

Additionally, tough-to-lyse samples, such as bacteria, yeast, and plant, can be processed with additional steps that enhance lysis. Cleanup of enzymatic reactions or purification of RNA from TRIzol® -extracted samples is also possible using this kit.

Purified RNA has high quality metrics, including A260/280 and A260/230 ratios >1.8, high RIN scores, and minimal residual gDNA. Captured RNA ranges in size from full-length rRNAs down to intact miRNAs. Additionally, differential binding conditions allow selective capture or exclusion of the sub-200 nucleotide RNA pool that includes miRNA, 5S rRNA, and tRNA. Purified RNA is suitable for downstream applications, such as RT-qPCR, cDNA synthesis, RNA-seq, Northern blot analysis.
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