Rosetta-gami™ B Competent Cells, Expression Strains, EMD Millipore

Supplier: MilliporeSigma

71135-3 71135-4 71136-3 71136-4 71137-3 71137-4

CA80601-670EA 410.5 CAD

CA80601-670 CA80601-672 CA80601-674 CA80601-676 CA80601-678 CA80601-680

Rosetta-gami™ B Competent Cells, Expression Strains, EMD Millipore

Competent Cells

Rosetta-gami B strains combines the key features of BL21 (and its Tuner™ derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm.

T7 lysozyme expression suppresses basal T7 expression
Compatible with ampicillin- or spectinomycin-resistant vectors

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

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