Toluidine Blue DNA Agar, HiMedia Laboratories
Supplier: HIMEDIA LABORATORIES PVT. CA
Toluidine blue DNA agar is used for detection of thermostable deoxyribonuclease activity.
DNA in the medium enables the detection of DNase activity which depolymerizes the DNA resulting in the formation of a clear zone around the microbial growth. Inclusion of toluidine blue aids in detection of DNase activity by the production of a visible bright rose-pink coloured reaction due to its metachromatic properties. Tris amino methane forms the buffering system. Sodium chloride and calcium chloride provides the ions and also maintains osmotic equilibrium.
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