Leukotriene C₄ ELISA Kit, Cayman Chemical Company

Supplier: Cayman Chemical Company

Leukotriene C₄ ELISA Kit, Cayman Chemical Company

Leukotriene C₄ ELISA Kit, Cayman Chemical Company

501070-480 501070-96

75816-848EA 3237.64 CAD

75816-848 75816-850

Leukotriene C₄ ELISA Kit, Cayman Chemical Company

Assays ELISAs

For the quantification of LTC₄ in culture media and other sample matrices.

Measure LTC₄ in tissue culture medium
Assay 24 samples in triplicate or 36 samples in duplicate
Measure LTC₄ levels down to 40 pg/mL
Incubation: 18 hours | Development: 90 – 120 minutes | Read: Colorimetric at 405 – 420 nm

Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid in eosinophils, mast cells, and macrophages. Their biosynthesis proceeds via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA₄ with 5(S)-HpETE as an intermediate. The conjugation of glutathione to LTA₄ results in the formation of LTC₄, which is then rapidly metabolized to LTD₄ and LTE₄. This metabolism is essentially complete within 10 minutes in the human lung. Cayman’s LTC₄ ELISA Kit is a competitive assay that can be used for quantification of LTC₄ in culture media and other sample matrices. The assay has a range from 17.1 – 5000 pg/mL and a sensitivity (80% B/B₀) value of approximately 40 pg/mL.

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