Anti-NCL Mouse Monoclonal Antibody (CF594) [clone: NCL/902]

Supplier: Biotium

BNC940902-100 BNC940902-500

75966-560EA 325.12 CAD

75966-560 75966-562

Anti-NCL Mouse Monoclonal Antibody (CF594) [clone: NCL/902]

Antibodies

This antibody recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®594 is a deep red fluorescent dye (Ex/Em 593/614 nm). It yields the brightest conjugates among spectrally similar dyes, and has excellent photostability.

Type: Primary
Antigen: NCL
Clonality: Monoclonal
Clone: NCL/902
Conjugation: CF594
Epitope:
Host: Mouse
Reactivity: Human
Isotype: IgG1, kappa

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