Pierce M-PER™, Mammalian Protein Extraction Reagents, Thermo Scientific

Supplier: Thermo Scientific
Pierce
78503 78505 78501 78501 78503 78505
CAPI78501EA 867.98 CAD
PI78505 CAPI78501 PI78501 CAPI78505 PI78503 CAPI78503
Pierce M-PER™, Mammalian Protein Extraction Reagents, Thermo Scientific
Buffers Extraction Buffers
M-PER™ Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

  • Gentle - mild detergent lysis, yielding extracts that are immediately compatible with Coomassie (Bradford) and BCA protein assays or SDS-PAGE
  • Compatible - extracts soluble proteins in non-denatured state, enabling direct use in immunoprecipitation and other affinity purification procedures
  • Amine-free and dialyzable - formulation ensures compatibility with subsequent assay systems
  • Convenient - lyse adherent cells directly in plate or after scraping and washing in suspension
  • Non-denaturing - maintain activity of luciferase, beta-galactosidase, CAT and other reporter genes as well or better than alternative methods

The complete cell lysis reagent dissolves cell membranes and extracts total cellular protein in only 5 minutes. This reagent requires little or no mechanical disruption and yields more protein than freeze/thaw cycles and sonication. This mammalian cell lysis reagent is so efficient that adherent cells do not need to be scraped from the culture dish, enabling easy and direct lysis and analysis of cells grown in 24-well and 96-well plates. Resulting cell lysates from either adherent and suspension cells are compatible with many downstream assays including immunoassays, enzyme assays and a variety of common reporter assays.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR