Hydrogen Peroxide Cell-Based Assay Kit, Cayman Chemical Company

Supplier: Cayman Chemical Company

Hydrogen Peroxide Cell-Based Assay Kit, Cayman Chemical Company

Hydrogen Peroxide Cell-Based Assay Kit, Cayman Chemical Company

600050-480 600050-96

75817-302EA 702.99 CAD

75817-302 75817-304

Hydrogen Peroxide Cell-Based Assay Kit, Cayman Chemical Company

Assays Cellular Assays

A fluorometric detection assay for hydrogen peroxide.

A simple fluorometric assay for the quantification of extracellular H₂O₂
Utilizes ADHP, a sensitive and stable probe for H₂O₂
Catalase, an H₂O₂ scavenger, is included as a control for specificity
Rapid assay; results in under 1 hour
Midpoint: 50% B/B₀: 100 – 300 pg/mL

Reactive oxygen species, such as hydrogen peroxide (H₂O₂) and superoxide are generated by phagocytes and participate in damaging invading miccroorganisms or other biologic targets. Cayman's H₂O₂ Cell-Based Assay Kit provides a simple fluorometric method for the sensitive quantitation of extracellular H₂O₂ produced by cultured cells. H₂O₂ is detected using ADHP (10-acetyl-3,7-dihydroxyphenoxazine), a highly sensitive and stable probe for H₂O₂. In the presence of horseradish peroxidase, ADHP reacts with H₂O₂ with a 1:1 stoichiometry to produce highly fluorescent resorufin. Resorufin fluorescence can be read using an excitation wavelength between 530 – 560 nm and an emission wavelength of 590 nm. Catalase, an H₂O₂ scavenger, is included in the kit for checking specificity of the assay.

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