Prostaglandin D₂ Express ELISA Kit, Cayman Chemical

Supplier: Cayman Chemical Company

Prostaglandin D₂ Express ELISA Kit, Cayman Chemical

Prostaglandin D₂ Express ELISA Kit, Cayman Chemical

512041-96 512041-480

75816-866EA 1002.79 CAD

75816-866 75817-276

Prostaglandin D₂ Express ELISA Kit, Cayman Chemical

Assays ELISAs

A competitive assay that can be used for quantification of PGD2

Measure PGD2 in multiple sample types
Assay 24 samples in triplicate or 36 samples in duplicate
Measure PGD2 levels down to 350 pg/ml
Rapid assay; get results in under 4 hours
Midpoint: 50% B/B₀: 800-1,500 pg/ml

Prostaglandin D2 (PGD2) is biosynthesized in the brain by soluble, glutathione-independent lipocalin-type PGD2 synthase. PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized in mast cells and leukocytes by a cellular, myeloid-type, glutathione-dependent PGD synthase. This PGD2 which is formed in the intracellular and vascular compartments is rapidly metabolized to 11β-PGF2α and other metabolites. Measurement of the parent eicosanoid PGD2 is appropriate in cell culture lysates and in CSF, where concentrations of several hundred pg/ml have been measured. Cayman’s PGD2 Express ELISA Kit is a competitive assay that can be used for quantification of PGD2 in cell culture lysates and enzymatic reactions. This ELISA offers the convenience of a fast assay with a two hour incubation time and one hour development time, while still achieving an assay range of approximately 117.2-15,000 pg/ml and a sensitvity (80% B/B0) of approximately 350 pg/ml.

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