The GlycoLink™ Immobilisation Kit contains affinity columns and reagents to immobilise glycoproteins through oxidised sugar groups for use in affinity-purification applications or studies of protein-protein interactions. This kit is ideal for purification of antigens using covalently coupled antibodies, determining binding partners for a glycoprotein of interest and for performing large-scale affinity purifications.
- High capacity with 1 to 10 mg of oxidised antibody or other glycoprotein per ml of resin
- Immobilise in as little as 30 minutes; achieve at least 90% coupling of most glycoproteins in less than 4 hours
- Hydrazide-activated UltraLink resin conjugates only to purified glycoproteins whose sugar (eg. sialic acid) groups have been gently oxidised with periodate
- Resonance structure of the hydrazone bonds are sufficiently stable for multiple affinity purifications with one batch of prepared resin; no stabilisation step required
- Immobilises IgG via the Fc region, thereby keeping both antigen binding sites available for capturing target
Antibodies immobilised by this hydrazide immobilisation chemistry have unobstructed antigen-binding sites and optimal purification capability. These columns can be regenerated and reused five times without loss in binding capacity.
Delivery information: Kit contains GlycoLink™ columns (1 ml, 10 columns), GlycoLink™ Coupling Buffer (2×100 ml), aniline (0.2 ml), Wash Buffer (120 ml), sodium meta-periodate (0.5 g), Zeba™ spin desalting columns (5 ml, 10 columns), PBS pack (makes 500 ml, 1 pack) and column accessories (10 each).
