Sephadex™ G-50 Gel Filtration Media, Cytiva

Supplier: Cytiva

17-0042-02 17-0043-02 17-0041-01 17-0042-01 17-0043-01
CA95016-774LEA 4571.58 CAD
CA95016-774L CA95016-778L CA95016-770L CA95016-772L CA95016-776L
Sephadex™ G-50 Gel Filtration Media, Cytiva
Protein Purification Protein Purification Resins

Sephadex G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight.


  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step
  • Suitable for purification of DNA from small molecules by gel filtration
  • Larger bead size for large scale group separations where high flow rates and low operating pressures are required
  • Classic gel filtration medium


Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex™ G-50 is one of six different G-types ranging from G-10 for small molecules to G-100 for very large molecules. Sephadex G-50 is available in 3 different particle sizes (Medium, Fine and Superfine). Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Medium is preferred for large scale group separations where high flow rates and low operating pressures are required.


The larger bead size of G-50 Medium is suited for large scale separations.


Ordering information: Supplied in lab packs.

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