Remove contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC preps without digesting the plasmid, cosmid, fosmid or BAC molecules
- Digest contaminating linear DNA without digesting nicked or closed-circular dsDNA or supercoiled DNA (plasmids, fosmids, etc.)
- Use as a final purification step for plasmid, cosmid, fosmid, and BAC vector and clone preparations
- Utilize whenever protecting circular dsDNA is important
Plasmid-Safe™ ATP-Dependent DNase selectively removes contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC clones or vector preparations
Plasmid-Safe ATP-Dependent DNase digests linear dsDNA to deoxynucleotides at slightly alkaline pH and, with lower efficiency, closed-circular and linear ssDNA. The enzyme has no activity on nicked or closed-circular dsDNA or supercoiled DNA. Therefore, Plasmid-Safe DNase is ideal as the final purification step for plasmid, cosmid, fosmid, and BAC vector and clone preparations.
Applications include:
Minimizes the possibility of cloning or sequencing contaminating chromosomal DNA from plasmid, cosmid, fosmid, or BAC preparations
Fast and easy protocol with minimal handling time
Complete protocols provided for using Plasmid-Safe DNase with miniprep, midiprep, and maxiprep plasmid, cosmid, fosmid, and BAC DNA purifications
Such preparations are frequently contaminated with fragments of bacterial genomic DNA generated during alkaline lysis. Other purification options, such as spin-columns or even CsCl centrifugation, do not effectively remove these contaminants and require further purification steps. Contaminating DNA fragments left behind by these methods ultimately can become ligated into a cloning vector, resulting in false positives and high backgrounds, or erroneous sequence data.
Certifications: For research use only. Not for human or diagnostic use.
Ordering information: Contents include enzyme, 10X reaction buffer and 25 mM ATP.
