Tri-Isolate RNA Pure Kit, IBI Scientific
Supplier: IBI Scientific
Cost-effective phenol, guanidine isothiocyanate plus spin column system for purification of high-quality total RNA.
Applications: cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting
- Cost effective
- Phenol, guanidine isothiocyanate solution plus spin column system
- No chlorophorm phase separation
- No isopropanol RNA precipitation
- No phenol carryover
- Sample Size: up to 5 x 10⁶ cultured cells, 10 – 50 mg tissue, up to 200 µl body fluids (blood, buffy coat, serum, plasma), 1 x 10⁹ bacterial cells, 20 – 50 mg plant tissue
- High quality RNA: A260/A280 > 1.8, A260/A230 > 1.8
IBI Tri-Isolate is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high quality total RNA from a variety of samples including cultured cells, tissue, body fluids, bacteria, and plant tissue
IBI Tri-Isolate is tested on a lot-to-lot basis. 10 µL from a 50 µL eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.
Initially, samples are homogenized in IBI Isolate Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash, and elute the high quality, total RNA in RNase-free water. The purified RNA can be used in a variety of downstream applications, including cDNA library construction, cloning, RT-PCR (End-point), Real-Time PCR, nuclease protection assays, northern blotting.
Certifications: For research and development purposes only.
Accessories information: Bacterial Lysis Kit is not included.
Caution: IBI Isolate contains phenol and guanidine isothiocyanate. During operation, always work in a fume hood, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask. Disposable/non-disposable glassware, plasticware and automatic pipettes should be sterile (RNase-free) and used only for RNA procedures.
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