ISP Medium No. 5, Glycerol Asparagine Agar Base, HiMedia Laboratories

Supplier: HIMEDIA LABORATORIES PVT. CA

M360-500G M360-2.5KG M360-5KG
CA61000-088EA 1830 CAD
CA61000-088 CA89225-292 CA89225-294
ISP Medium No. 5, Glycerol Asparagine Agar Base, HiMedia Laboratories
Media for Microbiology Dehydrated Media for Microbiology

Used for cultivation of Streptomyces species as per International Streptomyces project.


  • Provides consistent and reproducible characteristic features of Streptomyces
  • Dipotassium phosphate buffers the medium
  • Based on the formulation described by Shirling and Gottlieb


ISP Medium No


This medium provides consistent and reproducible characteristic features of Streptomyces. Glycerol serves as the carbon source while asparagine is the amino acid source for the growth of Streptomyces species. Trace mineral requirement of Streptomyces is satisfied by the trace salt solution, which contains various salts. Dipotassium phosphate buffers the medium.


5 (Glycerol Asparagine Agar Base) is based on the formulation described by Shirling and Gottlieb and is used for cultivation and characterization of Streptomyces species as recommended by the International Streptomyces Project. Being primarily soil inhabitants, Streptomyces are most commonly limited to causing actinomycotic mycetoma. Areas more prone to formation of mycetomas are those that are frequently traumatized or that come into contact with soil.

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