EnzyChrom™ Lactate Assay Kit, BioAssay Systems

Supplier: BioAssay Systems
EDLC-100
75878-082EA 1033.86 CAD
75878-082
EnzyChrom™ Lactate Assay Kit, BioAssay Systems
Assays Cellular Assays
For quantitative colorimetric determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.

  • Linear detection range 0.05 - 2 mM D-lactate in 96-well plate assay
  • Convenient room temperature assay. No 37°C heater is needed
  • Can be readily automated to process thousands of samples per day

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready. Procedures for measuring lactate concentration are very desirable. BioAssay Systems’ EnzyChrom™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.
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