innuPREP RNA Mini Kit, Analytik Jena
Supplier: Analytik Jena CA
The innuPREP RNA Mini Kit is a jack-of-all-trades when it comes to extracting total RNA that is excellent in terms of both quality and quantity.
- Fast, efficient purification of total RNA from a wide variety of different starting materials and in varying amounts
- Prefiltration to selectively remove genomic DNA with no DNase digestion
- Eliminates the need for using highly toxic β-mercaptoethanol
- Ready-to-use RNA isolated after only 15-40 minutes; quality and quantity of isolated RNA is excellent
The innuPREP RNA Mini Kit is a jack-of-all-trades when it comes to extracting total RNA that is excellent in terms of both quality and quantity. The specially optimized lysis buffer system guarantees isolation of intact RNA and lasting deactivation of endogenous and exogenous RNases. A pre-column contained in the kit can be used to remove genomic DNA, which virtually eliminates the traditional need for DNase I digestion. The RNA is then bound to a second spin filter membrane, washed and finally eluted in 30-80µl of RNase-free water. As such, the extraction process can be concluded in 15-40 minutes, depending on the starting material.
Specifications
Starting material: Eukaryotic cells (max. 5 × 10^6); Tissue samples (max. 20mg); Gram+ and gram– bacteria (max. 1 × 10^9); Biopsie
Column binding capacity: approx. 100µg RNA
Average purity: 1.8 – 2.1
Extraction time: Approx. 15 - 40 minutes
Average yield: Up to 100µg RNA, depending on the type and quantity of starting material
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