Human Recombinant TNF-A (Animal free) (from E. coli)

Supplier: FUJIFILM IRVINE SCIENTIFIC, INC

100-111AF-10UG 100-111AF-1MG 100-111AF-50UG 100-111AF-500UG
10787-186EA 151.36 CAD
10787-186 10787-188 10787-190 10821-848 10822-398 77660-656
Human Recombinant TNF-A (Animal free) (from E. coli)
Proteins and Peptides

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS).


  • High quality
  • Low endotoxin
  • Affordable


TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.


Caution: For research use only.

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SPECIFICATIONS

Protein/peptide name TNF-A (AF) TNF-A (AF) TNF-A (AF)
Environmentally Preferable
Protein synonyms DIF|cachexin|cachectin|necrosin|TNFSF2|TNF|cytotoxin DIF|cachexin|cachectin|necrosin|TNFSF2|TNF|cytotoxin DIF|cachexin|cachectin|necrosin|TNFSF2|TNF|cytotoxin
Protein/peptide type Recombinant Recombinant Recombinant
Protein Function Cytokine Cytokine Cytokine
Species Human Human Human
Source E. coli E. coli E. coli
Conjugation Unconjugated Unconjugated Unconjugated
Gene ID P01375 P01375 P01375
Sequence MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Protease-free N N N
Animal-free Y Y Y
Nuclease-free N N N
Carrier-free Y Y Y
Endotoxin-free N N N
Endotoxin level Low Low Low
Endotoxin content Endotoxin LAL (EU/µg) ≤1 Endotoxin LAL (EU/µg) ≤1 Endotoxin LAL (EU/µg) ≤1
UniProtKB P01375 P01375 P01375
Biological activity Bioactivity assay: Cytolysis of mouse L929 cells in the presence of actinomycin D, ED50 ≤2 ng/ml, bioactivity ≥5.0×10⁵ units/mg Bioactivity assay: Cytolysis of mouse L929 cells in the presence of actinomycin D, ED50 ≤2 ng/ml, bioactivity ≥5.0×10⁵ units/mg Bioactivity assay: Cytolysis of mouse L929 cells in the presence of actinomycin D, ED50 ≤2 ng/ml, bioactivity ≥5.0×10⁵ units/mg
Purity ≥95% ≥95% ≥95%
Grade RUO RUO RUO
Formulation 10 mM sodium phosphate, pH 7.5 10 mM sodium phosphate, pH 7.5 10 mM sodium phosphate, pH 7.5
Molecular weight 17.5 kDa 17.5 kDa 17.5 kDa
Reconstitution instructions Sterile water at 0.1 mg/ml Sterile water at 0.1 mg/ml Sterile water at 0.1 mg/ml
Storage conditions −20 °C −20 °C −20 °C
Shipping temperature RT RT RT
Tested applications Bioassay Bioassay Bioassay

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