EnGen® Spy Cas9 NLS, New England Biolabs

Supplier: New England Biolabs

EnGen Cas9 NLS, S. pyogenes

EnGen Cas9 NLS, S. pyogenes

M0646M M0646T M0646M M0646T

CA103218-920EA 914 CAD

103218-920 CA103218-920 103218-918 CA103218-918

EnGen® Spy Cas9 NLS, New England Biolabs

Protein Purification Protein Purification Reagents

EnGen Spy Cas9 NLS, is an RNA-guided endonuclease that catalyzes site- specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. EnGen Spy Cas9 NLS contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein.

Ideal for direct introduction of Cas9/sgRNA complexes
Dual NLS for improved transport to the nucleus
Compatible with EnGen® sgRNA Synthesis Kit, S. pyogenes and the EnGen Mutation Detection Kit

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