Pierce™ Recombinant Protein A/G (from E. coli)

Supplier: Thermo Scientific

32490

CAPI32490EA 512.43 CAD

CAPI32490

Pierce™ Recombinant Protein A/G (from E. coli)

Proteins and Peptides

Pierce™ Recombinant Peroxidase Conjugated (HRP-Horseradish Peroxidase) Protein A/G is useful to probe and detect antibodies, especially IgG isotypes, of many different species hosts in Western blotting, ELISA, IHC and other immunoassay protocols.

Contains four Fc-binding domains from Protein A and two from Protein G
Provides binding for all antibody species and subclasses recognised by either Protein A or Protein G

The extended Fc-binding properties of Protein A/G make it a tool in the investigation and purification of immunoglobulins. Protein A/G binds to all human IgG subclasses, IgA, IgE, IgM and to a lesser extent IgD; however, it does not bind mouse IgA, IgM or murine serum albumin. Protein A/G is an excellent tool for purification and detection of mouse monoclonal antibodies from IgG subclasses without interference from these other serum proteins. Individual subclasses of mouse monoclonal are most likely to have stronger affinity to this chimeric protein than to either Protein A or Protein G.

Form: salt-free powder
A280 of 0.1% solution:

0.505
Isoelectric point (pI): 4.65

Caution: For Research Use Only. Not for use in diagnostic procedures.

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