Live-or-Dye™ Fixable Viability Staining Kits, Biotium

Supplier: Biotium

32007 32013 32009-T 32009 32012-T 32003-T 32006-T 32004 32011 32002 32002-T 32008 32008-T 32005 32003 32005-T 32007-T 32004-T 32013-T 32012 32006 32011-T
10018-180EA 497.89 CAD
76403-952 76403-946 76403-948 10018-180 10018-170 10018-182 10018-380 10018-172 10018-370 76407-416 10018-178 10018-376 76407-414 10018-168 10018-378 76403-950 10018-382 10018-372 10018-174 10018-384 10018-374 10018-176
Live-or-Dye™ Fixable Viability Staining Kits, Biotium
Assays Cellular Assays
Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry or microscopy.

  • Highly dead-cell specific
  • Covalent labeling with reactive dyes
  • Withstand fixation and permeabilization
  • The most available colors
  • Dye options designed for spectral flow

Live-or-Dye™ Fixable Viability Stains are cell membrane impermeable amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. On live cells, the dyes react with surface proteins, but these are much less abundant than intracellular proteins, resulting in low staining levels compared to dead cells. Live-or-Dye™ labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.

Live-or-Dye™ Fixable Viability Stains are cell membrane impermeable amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. On live cells, the dyes react with surface proteins, but these are much less abundant than intracellular proteins, resulting in low staining levels compared to dead cells. Live-or-Dye™ labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.

The dyes are named for their excitation/emission wavelengths. Choose from a wide variety of colors with fluorescence from UV to near-IR. Live-or-Dye™ 488/515, 568/583, 594/614, & 640/662 can be used for either flow cytometry or fluorescence microscopy. Live-or-Dye™ 510/550 & 665/685 have been validated for use in spectral flow cytometry on the Cytek® Aurora. Live-or-Dye™ 787/808 offers the best performance for the 808 laser line, effectively freeing up the channels in the visible spectrum.

Kit Components:
Live-or-Dye™ Fixable Viability Dye
Anhydrous DMSO

Ordering information: Number of assays is based on 1 mL reaction size (106cells); actual number of assays may vary depending on staining volume.
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