Mag-Bind® Ultra-Pure Plasmid DNA Kit, Omega Bio-Tek
Supplier: OMEGA BIO-TEK CA
The Mag-Bind® Ultra-Pure Plasmid DNA 96 Kit combines the power of Mag-Bind® technology with the innovative ETR technology to deliver high quality endotoxin free plasmid DNA in high throughput format.
- Endotoxins efficiently reduced (<0,1 EU/µg)
- Fast high throughput procedure
- Simple protocol can be automated without sacrificing DNA quality
- Paramagnetic particles enable rapid isolation of plasmid DNA
Yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 1 mL of overnight culture in LB medium typically produces 10-15 μg for high copy plasmid. Nucleic acids are easily eluted with deionized water or low salt buffer. The purified plasmid can be used directly for automated fluorescent DNA sequencing, such as with BigDye sequencing chemistry, transfection as well as for other standard molecular biology techniques including restriction enzyme digestion.
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