Medium 199 (For Suspension Culture) Mammalian Cell Culture Medium, HiMedia

Supplier: HIMEDIA LABORATORIES PVT. CA

AT014-5L AT014-20L
CA95016-530EA 252.93 CAD
CA95016-530 CA95016-532 CA95016-534 CA95016-536 CA95016-538 CA95016-540 CA95016-542 CA95016-544 CA95016-546 CA95016-548 CA95016-550 CA95016-552
Medium 199 (For Suspension Culture) Mammalian Cell Culture Medium, HiMedia
Cell Culture Media

Medium 199 was the first nutritionally defined medium developed by Morgan, Morton and Parker in 1950.


  • Formulated for nutritional studies on primary chick embryo fibroblasts in the absence of any additives
  • Formulated with either Hank’s salts or Earle’s salts
  • Can be used for growth of a wide variety of cells


This complex medium was formulated specifically for nutritional studies on primary chick embryo fibroblasts in the absence of any additives. It was observed that explanted tissue could survive in Medium 199 without serum but long term cultivation of cells required supplementation of the medium with serum. Media 199 are formulated with either Hank’s salts or Earle’s salts. The medium when supplemented with serum can be used for growth of a wide variety of cells. Media 199 are presently used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells.

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