The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.
- Application: Flow Cytometry
Contents:
The APO-DIRECT Kit is shipped in one container that houses two packages. One package is shipped at ambient temperature and should be stored at 2-8° C upon arrival. The other package is in styrofoam, containing frozen ice packs. The contents of this styrofoam container should be stored at -20° C upon arrival. We have determined this shipping method is adequate to maintain the integrity of the kit components. Upon arrival store the reagents at the appropriate temperatures. Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 50 cell suspensions. Included are 5 mL positive and 5 mL negative control cell suspensions of approximately 1 x 106 cells per ml in 70% (v/v) ethanol. The control cells are derived from a human lymphoma cell line and have been fixed as described in the fixation procedure.
Delivery information: The APO-DIRECT Kit is shipped in one container and consists of two packages. Upon arrival one should be stored at 2-8 °C and the other at -20 °C.
