Pierce™ HisPur™ Affinity Chromatography Cartridges, Cobalt, Thermo Scientific

Supplier: Thermo Scientific

90093 90094

CAPI90093PK 598.04 CAD

CAPI90093 CAPI90094

Pierce™ HisPur™ Affinity Chromatography Cartridges, Cobalt, Thermo Scientific

Chromatography Columns

Pierce™ HisPur™ cobalt chromatography cartridges are convenient, ready-to-use pre-packed devices for efficient purification of polyhistidine-tagged proteins from bacterial, mammalian and baculovirus-infected cells.

Binds at least 10 mg of purified 6 x His-tagged protein per ml of resin
Cobalt-chelate coordination core binds fewer host protein contaminants, resulting in lower background than nickel resins
Low metal leaching means no metal contamination in eluted histidine-tagged protein sample

HisPur™ cobalt chromatography cartridges are compatible with the major automated liquid-chromatography systems or manual syringe processing. The cartridges attach directly to ÄKTA™ or FPLC Systems without additional connectors. The included accessory pack readily adapts cartridges for use with Luer-Lok® syringe fittings or ¹/₁₆" tubing. These cartridges enable fast, easy and reproducible chromatographic separations and can be regenerated for multiple uses.

His-tagged proteins are purified from total soluble protein extract using a cobalt-charged tetradentate chelator immobilized onto 6% crosslinked agarose.

Delivery information: Kit contains an accessory pack (1 female Luer-Lok® Adapter, 1 connector fitting, 1 column plug and 1 or 2 bottom caps).

Caution: For research use only. Not for use in diagnostic procedures.

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