The Thermo Scientific 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems.
- High Expression with Up to 750 µg/ml of Expressed Protein
- Low Variability between Experiments
- Express High Levels of Protein with 6 Hours to Overnight Incubation
- Obtain Functionally Active Proteins, Including those Containing Disulfide Bonds
- Transcription and Translation is Performed in One Reaction Step
- Amenable to Incorporation of Heavy or Unnatural Amino Acids
The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human translation machinery and generate functional full-length proteins. In this system, protein expression is performed in a proprietary dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression in an overnight incubation of up to 750 µg/ml. The complete mini-scale kit includes all the components required for transcription and translation of a recombinant gene, including an optimised expression vector.
Delivery information: Each 1- Step Human High-Yield Mini IVT Kit contains HeLa lysate (110 or 500 µl), accessory proteins (25 or 100 µl), reaction mix (40 or 200 µl), 5X dialysis buffer (600 µl or 3 ml), positive control DNA (pCFE-GFP - 10 µg), cloning vector (pT7CFE1-NHis-GST-CHA - 10 µg), microdialysis devices (2 or 10), and nuclease-free water (5 ml or 3×5 ml).
Caution: For Research Use Only. Not for use in diagnostic procedures.