Pierce™, 1-Step™ Human High-Yield Mini IVT Kit

Supplier: Thermo Fisher Scientific
Pierce
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CAPI88891EA 5137.21 CAD
CAPI88891 CAPI88890 PI88891 PI88890
Pierce™, 1-Step™ Human High-Yield Mini IVT Kit
Protein Expression Protein Expression Systems
The Thermo Scientific 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems.

  • High Expression with Up to 750 µg/ml of Expressed Protein
  • Low Variability between Experiments
  • Express High Levels of Protein with 6 Hours to Overnight Incubation
  • Obtain Functionally Active Proteins, Including those Containing Disulfide Bonds
  • Transcription and Translation is Performed in One Reaction Step
  • Amenable to Incorporation of Heavy or Unnatural Amino Acids

The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human translation machinery and generate functional full-length proteins. In this system, protein expression is performed in a proprietary dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression in an overnight incubation of up to 750 µg/ml. The complete mini-scale kit includes all the components required for transcription and translation of a recombinant gene, including an optimised expression vector.

Delivery information: Each 1- Step Human High-Yield Mini IVT Kit contains HeLa lysate (110 or 500 µl), accessory proteins (25 or 100 µl), reaction mix (40 or 200 µl), 5X dialysis buffer (600 µl or 3 ml), positive control DNA (pCFE-GFP - 10 µg), cloning vector (pT7CFE1-NHis-GST-CHA - 10 µg), microdialysis devices (2 or 10), and nuclease-free water (5 ml or 3×5 ml).

Caution: For Research Use Only. Not for use in diagnostic procedures.
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