Trifluoroacetic acid ≥99.5% for LC-MS, Pierce™
Supplier: Thermo Scientific
Synonyms:
TFA
Thermo Scientific Pierce™ Trifluoroacetic Acid (TFA), Sequencing grade is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.
- High purity and exceptional clarity
- High-performance packaging
- Economical convenience
Trifluoroacetic acid (TFA) is the most commonly used ion pairing agent for use in reverse-phase HPLC peptide separations because it sharpens peaks and improves resolution, is volatile and easily removed, has low absorption within detection wavelengths. It is manufactured to the highest specifications to ensure the integrity of your data, maximize sensitivity in your assay and to prolong the life of your equipment.
Making 0.1% Trifluoracetic Acid Solutions:
For complex peptide separations, the key to success can be to vary selectivity. Varying mobile phase composition on the same column can change selectivity enough to resolve peptides that would otherwise overlap. Trifluoroacetic acid is the most frequently used modifier for peptide separations in reverse-phase HPLC. The TFA concentration usually specified is 0.1%. For reproducible separations from run-to-run or from lab-to-lab, it is essential to make TFA concentrations the same.
Trifluoroacetic acid concentration can and should be specified as either "w/v" (weight/volume), or as "v/v" (volume/volume). The w/v specification designates that the TFA is to be weighed and added to a volume of mobile phase (e.g. 0.1% TFA w/v requires one gram of TFA per liter). The v/v specification designates that the TFA is to be measured by volume (e.g. 0.1% TFA v/v requires one mL of TFA per liter).
Because the density of trifluoroacetic acid is 1.53 g/ml the difference between 0.1% TFA (w/v) and 0.1% TFA (v/v) is more than 50%. For the sake of reproducibility, it is essential for authors of a method to specify, and for users of a method to know, whether the TFA concentration is given as "w/v" or "v/v".
Caution: For Research Use Only. Not for use in diagnostic procedures.
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Formula:
C₂HF₃O₂ MW: 114.02 g/mol Boiling Pt: 71.8 °C (1013 hPa) Melting Pt: –15 °C Density: 1.490 g/cm³ (20 °C) |
MDL Number:
MFCD00004169 CAS Number: 76-05-1 UN: 2699 ADR: 8,I |
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