Thermo Scientific Pierce LDS Sample Loading Buffer (4X) is a nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes.
Features of LDS Sample Loading Buffer:
Concentrated—4X formulation provides more versatility than traditional 2X buffers
Nonreducing—ready to use for non-reducing SDS-PAGE, or the preferred type and amount of reducing agent (e.g., DTT) can be added to produce reducing conditions
SDS alternative—lithium dodecyl sulfate (LDS) replaces sodium dodecyl sulfate (SDS) of traditional loading buffers but functions equally well
Physiologic pH—buffered with triethanolamine (pH 7.6) rather than Tris (pH 6.8)
Lane marker dyes—includes trace amounts of phenol red and coomassie G250 as visible markers of the dye front
Convenient—stable at room temperature, enabling storage at the bench where electrophoresis is performed
LDS Sample Buffer, Non-Reducing (4X) is a convenient sample buffer for use in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The buffer contains coomassie dye, enabling visualization of the electrophoretic progress by the location of the dye front. The LDS Sample Buffer, Non-Reducing (4X) may be used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.
Concentrated—4X formulation provides more versatility than traditional 2X buffers
