IPTG (isopropyl-β-D-thiogalactopyranoside), powder, Pierce™
Supplier: Thermo Scientific
Synonyms:
(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol
, IPTG
, Isopropyl Beta-D-1-thiogalactopyranoside
Thermo Scientific Pierce IPTG is isopropyl-β-D-thiogalactopyranoside for use with beta-galactosidase substrates such as X-Gal to detect expression of vectors, plasmids, or DNA fragments that contain the reporter gene.
- X-Gal yields an intense blue precipitate to detect β-galactosidase expression
- Can be combined with IPTG to selectively maximize the detection of β-galactosidase
The reagent X-Gal (5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase that yields a blue precipitate, is soluble in DMF, and can be used to detect vectors, plasmids, or DNA fragments that contain the β-Galactosidase gene.
IPTG (Isopropyl-β-D-thiogalactopyranoside) may be used to maximize the expression of genes in expression vectors. When screening a lambda bacteriophage library with an antibody, the probability of success can be increased by preventing the expression of the fusion protein until the plaques are well established. By placing a nitrocellulose filter containing the inducer IPTG onto the plate and continuing growth at 37˚C, the expression of the potential β-galactosidase-cDNA fusion protein can be induced after 3-4 hours of plaque growth. The nitrocellulose filter is then screened with antibodies. IPTG is also used in combination with X-Gal to detect active β-galactosidase and is practical in applications using bacteriophage M13 and Igt11 vectors.
Caution: For Research Use Only. Not for use in diagnostic procedures.
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Formula:
C₉H₁₈O₅S MW: 238.31 g/mol |
MDL Number:
MFCD00063273 CAS Number: 367-93-1 |
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