Alkaline Phosphatase

Supplier: Thermo Scientific

31391 31392

CAPI31391EA 2859.77 CAD

CAPI31391 CAPI31392

Alkaline Phosphatase

Enzymes

This purified calf intestinal alkaline phosphatase (CIP) is supplied in Tris buffer and 50% glycerol for use in protein research methods.

Purified form, ready to dilute and conjugate without prior dialysis
Concentrated approximately 20 mg/mL
High specific activitywhich is typically greater than 1600 units/mg
Tris buffered solution supplied in 5 mM Tris, 5 mM magnesium chloride and 0.1 mM zinc chloride
pH ~7.0 in 50% glycerol

The main applications for alkaline phosphatase in molecular biology and protein research are to remove 5'-phosphate groups from DNA or as a reporter system for immunoassays such as ELISA

One unit equals the amount of protein required to hydrolyze one micromole of p-nitrophenyl phosphate (PNPP) per minute at 25°C in a glycine buffer, pH 9.6.

For these latter methods, the enzyme is usually conjugated to specific primary or secondary antibodies and its activity is detected with a color-forming (or light-generating) substrate.

Caution: For Research Use Only. Not for use in diagnostic procedures.

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