Pierce™ HABA (4'-hydroxyazobenzene-2-carboxylic acid), Thermo Scientific

Supplier: Thermo Fisher Scientific
Pierce
28010 28010
CAPI28010EA 423.15 CAD
CAPI28010 PI28010
Pierce™ HABA (4'-hydroxyazobenzene-2-carboxylic acid), Thermo Scientific
Protein Purification Protein Purification Reagents
Pierce™ HABA (4'-hydroxyazobenzene-2-carboxylic acid), a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeled proteins and other molecules.

  • HABA-avidin complex can be used over a wide range of pH and salt concentrations
  • Amount of Avidin can be calculated directly from the increased absorbance at 500nm complexing with the HABA Dye
  • Calculate results directly from absorbance values based on extinction coefficients using the procedure outlined in the instructions

Determine the molar ratio of biotin incorporated into a protein using the HABA-Avidin method. HABA dye (4'-hydroxyazobenzene-2-carboxylic acid ) binds to avidin to produce a yellow-orange colored complex which absorbs at 500nm. Free biotin will displace the HABA dye and cause the absorbance to decrease. A standard curve can be established using the free biotin to estimate the number of moles of biotin incorporated after biotinylating a protein.

Caution: For Research Use Only. Not for use in diagnostic procedures.
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