Pierce™ Rapid Mouse Antibody Isotyping Kit, Thermo Scientific
Supplier: Thermo Scientific
The Pierce™ Rapid Isotyping Kit is a 5-minute lateral-flow assay with ELISA sensitivity for class and subclass determination of monoclonal antibodies.
- Stable for at least one year at room temperature
- Assay does not cross-react with foetal bovine serum
- Detect antibodies in tissue culture media or ascites fluid samples at concentration greater than 10 ng/mL
- Results are similar to the standard ELISA-based isotyping assays
This antibody isotyping kit uses cartridges that provide a color-readout of the monoclonal antibody isotype within five minutes after pipetting a small amount of diluted culture supernatant or ascites fluid sample into the sample well
The assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to the well of the cassette. The gold conjugates embedded in the cassette form specific class and subclass soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated membrane. Results are displayed as a red band indicating the antibody isotype or subclass.
The kit determines mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgM.
Delivery information: The kit includes isotyping cassettes (10 pouches containing 2 or 3 cassettes) and sample diluent (45 mL). The standard kit contains a cassette with a strip impregnated with anti-IgG1, anti-IgG2a and anti-IgG2b antibodies and a cassette with a strip impregnated with anti-IgG3, anti-IgA and anti-IgM antibodies. The kappa and lambda kit also includes a cassette impregnated with anti-kappa and anti-lambda antibodies. Each cassette contains a control band.
Caution: For Research Use Only. Not for use in diagnostic procedures.
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