Pierce™ Avidin Agarose, Thermo Scientific

Supplier: Thermo Scientific

Pierce™ Avidin Agarose, Thermo Scientific

Pierce™ Avidin Agarose, Thermo Scientific

20219 20225

CAPI20219EA 712.47 CAD

CAPI20219 CAPI20225

Pierce™ Avidin Agarose, Thermo Scientific

Protein Purification Protein Purification Resins

Thermo Scientific Pierce Avidin Agarose can be used in a variety of applications for the affinity purification of biotinylated macromolecules.

Strong, nearly irreversible biotin:avidin interaction
Approximate Binding Capacity>20 µg biotin/mL resin

In one variation, an antibody that has an affinity for a particular antigen is labeled with biotin. Cells containing the antigen are lysed, then incubated with the biotinylated antibody to form a typical antigen/antibody complex. To isolate the antigen, the crude mixture is passed through an immobilized avidin or streptavidin column, which will bind the complex. After appropriate washes, the antigen can then be eluted from the column with a pH 2.8 elution buffer. The biotinylated antibody is retained by the column.

Caution: For Research Use Only. Not for use in diagnostic procedures.

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