Pierce™ Red Firefly Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific
Supplier: Thermo Scientific
Red Firefly Luc vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.
- Intracellular Red Firefly luciferase gene, optimised for high expression in mammalian systems
- Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
- Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimise background by reducing transcriptional read-through
- Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells respectively
- High-copy pUC bacterial DNA replication origin
pMCS-Red Firefly Luc is a multiple cloning site plasmid designed to accept a promoter sequence for the study of gene regulation using the intracellular red firefly luciferase reporter with excellent light intensity.
pCMV-Red Firefly Luc is a constitutive expression vector having the luciferase gene under the CMV (Cytomegalovirus) promoter. Strong (CMV) constitutive promoters for co-transfection and normalisation.
pTK-Red Firefly Luc is a constitutive expression vector having the luciferase gene under the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter. Weak (TK) constitutive promoters for co-transfection and normalisation.
Caution: For Research Use Only. Not for use in diagnostic procedures.
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