NovaTaq™ Hot Start DNA Polymerase, MilliporeSigma

Supplier: MilliporeSigma

71091-3 71091-4
CA80511-392EA 2023.55 CAD
CA80511-392 CA80511-394
NovaTaq™ Hot Start DNA Polymerase, MilliporeSigma
Nucleic Acid Reagents End-point PCR Enzymes and Kits

NovaTaq™ Hot Start DNA Polymerase is a chemically modified form of Taq DNA polymerase that is inactive at ambient temperature.


  • Highly specific, thereby minimizes the non-specific amplifications
  • Improved low-copy target amplification
  • Ambient temperature setup compatible with automation
  • Target amplification of up to 5 kbp
  • Ideal for quantitative and high-throughput PCR applications


The enzyme provides improved specificity when compared to standard Taq DNA polymerase and can minimize the generation of nonspecific amplification products, such as primer-dimers and misprimed products.


The enzyme must be activated by heat treatment (7 - 10 min at 95 °C), after which thermal cycling can proceed. The enzyme generates PCR products with 3′-nucleotide overhangs, suitable for cloning with a varierty of vector kits.

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