NovaTaq™ Hot Start DNA Polymerase, MilliporeSigma
Supplier: MilliporeSigma
NovaTaq™ Hot Start DNA Polymerase is a chemically modified form of Taq DNA polymerase that is inactive at ambient temperature.
- Highly specific, thereby minimizes the non-specific amplifications
- Improved low-copy target amplification
- Ambient temperature setup compatible with automation
- Target amplification of up to 5 kbp
- Ideal for quantitative and high-throughput PCR applications
The enzyme provides improved specificity when compared to standard Taq DNA polymerase and can minimize the generation of nonspecific amplification products, such as primer-dimers and misprimed products.
The enzyme must be activated by heat treatment (7 - 10 min at 95 °C), after which thermal cycling can proceed. The enzyme generates PCR products with 3′-nucleotide overhangs, suitable for cloning with a varierty of vector kits.
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