Two parallel blots were incubated with primary antibodies for an expressed protein, then incubated with Anti-Mouse IgG AP Conjugate and processed using chemiluminescent detection.
- Detect markers simply by adding the S-protein (AP or HRP Conjugate) or HisTag® Monoclonal Antibody to the same incubation used for sample detection
- S-protein conjugate does not interfere with antibodies or streptavidin detection
- Can be used with colorimetric and chemiluminescent AP or HRP substrates
- Recombinant, unmodified markers give sharp, accurately sized bands
- Markers are supplied at the working dilution in gel loading buffer; concentration optimized for Western detection
- Protein sizes are 15, 25, 35, 50, 75, 100, and 150 kDa.
The S-protein AP Conjugate was included with secondary antibody for the blot on the left. The lane order was as follows:
Lane 1 - 5 µl Perfect Protein western markers
Lane 2 - 10 ng induced cell extract
Lane 3 - 1 µg uninduced cell extract
The other blot contains the Perfect Protein western markers and purified GUS expressed in insect cells incubated with a GUS-specific monoclonal antibody, then incubated with Anti-Mouse IgG HRP Conjugate and S-protein HRP Conjugate and detected with chemiluminescent HRP substrate. The blot lane order was:
Lane 1 - 5 µl Perfect Protein western markers
Lane 2 - 800 ng purified GUS protein
