Perfect Protein™ Western Blotting Kit, MilliporeSigma

Supplier: MilliporeSigma

69078-3 69959-3 69959-3 69078-3

CA80030-586EA 230.6 CAD

CA80030-586 EM69078-3 80030-586 CA80031-084

Perfect Protein™ Western Blotting Kit, MilliporeSigma

Blotting Kits

Two parallel blots were incubated with primary antibodies for an expressed protein, then incubated with Anti-Mouse IgG AP Conjugate and processed using chemiluminescent detection.

Detect markers simply by adding the S-protein (AP or HRP Conjugate) or HisTag® Monoclonal Antibody to the same incubation used for sample detection
S-protein conjugate does not interfere with antibodies or streptavidin detection
Can be used with colorimetric and chemiluminescent AP or HRP substrates
Recombinant, unmodified markers give sharp, accurately sized bands
Markers are supplied at the working dilution in gel loading buffer; concentration optimized for Western detection
Protein sizes are 15, 25, 35, 50, 75, 100, and 150 kDa.

The S-protein AP Conjugate was included with secondary antibody for the blot on the left. The lane order was as follows:
Lane 1 - 5 µl Perfect Protein western markers
Lane 2 - 10 ng induced cell extract
Lane 3 - 1 µg uninduced cell extract

The other blot contains the Perfect Protein western markers and purified GUS expressed in insect cells incubated with a GUS-specific monoclonal antibody, then incubated with Anti-Mouse IgG HRP Conjugate and S-protein HRP Conjugate and detected with chemiluminescent HRP substrate. The blot lane order was:
Lane 1 - 5 µl Perfect Protein western markers
Lane 2 - 800 ng purified GUS protein

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