Pellet Paint® Co-Precipitant, MilliporeSigma
Supplier: MilliporeSigma
Pellet Paint® Co-Precipitant is a visible dye-labeled carrier formulated specifically for use in alcohol precipitation of nucleic acids.
- Compatible with most molecular biology procedures and is free of contaminating nucleic acids and nucleolytic enzymes
- Suitable for the rapid, quantitative precipitation of DNA and RNA, including PCR clean up and template preparation
- Compatible with automated Cy5® sequencers
- Although it absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible
The two-minute precipitation uses just 2 µl per reaction and requires no low-temperature incubations or prolonged centrifugation
Most applications of PCR benefit from a clean-up step in which primers and other reactants are removed and the target DNA is concentrated. Pellet Paint Co- Precipitant is ideal for this cleanup because the procedure is rapid, primers < 50 nt in length are efficiently removed, and the DNA is quantitatively recovered. Furthermore, it is easy to tell when the DNA has been completely resuspended following the precipitation step.
Both RNA and DNA are efficiently precipitated from even dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink color. The pellet can be easily followed during washing steps and prevents losses during handling.
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