Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans Golgi network to lysosomes. Together with medium and small subunits, adaptins form a heterotetrameric complex called an adaptor whose role is to promote the formation of clathrin-coated pits and vesicles and to recognise sorting signals within the cytosolic tails of transmembrane cargo molecules. Gamma-adaptin protein belongs to the adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network and/or endosomes. SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane.
This antibody has been shown to be specific for AP-1
Application Information:
IHC, WB. This antibody works in immunohistochemistry on frozen or wax embedded tissue. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions are 1: 200 to 1: 2000 depending on tissue and detection method. For western blotting, 1: 1000 to 1: 4000 is recommended depending on detection method. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: AP1G1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: amino acids 5-24 of Human AP-1
Host: Rabbit
Isotype: IgG
Reactivity: Human, Rat
