IBI DNA/RNA/Protein Extraction Kit, IBI Scientific

Supplier: IBI Scientific

IB47700 IB47701 IB47702
10304-254PK 63.22 CAD
10304-254 10304-256 10304-258
IBI DNA/RNA/Protein Extraction Kit, IBI Scientific
Nucleic Acid Reagents Nucleic Acid Purification Kits and Reagents

The DNA/RNA/Protein Extraction Kit provides an efficient method for purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids


DNA/RNA purification can be completed in 25 minutes without phenol/chloroform extraction or alcohol precipitation, and protein purification can be completed in 50 minutes. The purified DNA, with approximately 20 – 30Kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-Time PCR, northern blotting, primer extension, mRNA selection, and cDNA synthesis. The purified proteins can be directly analyzed on a SDS-PAGE and subsequent western blot.


The quality of the DNA/RNA Extraction Kit is tested on a lot-to-lot basis by isolating genomic DNA and total RNA from 1×106 cultured animal cells. The purified DNA and total RNA is quantified with a spectrophotometer and analyzed by electrophoresis on a 1% agarose gel.  The purified protein is quantified by Bradford assay analyzed on SDS-PAGE.


Chaotropic salt is used to lyse cells and inactivate DNases and RNases, allowing DNA to bind to the genomic DNA spin column. The flow-through can then be transferred to the RNA Spin column for RNA binding. Contaminants are effectively removed using wash buffers followed by pure genomic DNA elution in a low salt buffer and pure total RNA elution in RNase-free water.

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SPECIFICATIONS

Environmentally Preferable
Application
Target molecule
Refine target molecule DNA,Total RNA,Total protein
Sample type Blood,Cultured cells
Method / Format
Prep size Midi
Sample size Cultured animal cells (up to 5x10^6), up to 25mg of tissue, up to 500 µl of whole human blood, up to 200 µl of biological liquids (serum, plasma)
Yield up to 9 µg of Genomic DNA from 1.5x10^6 HeLa cell
20 µg of Total RNA from 1.5x10^6 HeLa cells
120 µg of protein from 1.5x10^6 HeLa cells
Operation tiime: 25 minutes (DNA/RNA Extraction); 50 minutes (Protein Extraction)

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