ProtoScript II First Strand cDNA Synthesis Kit, New England Biolabs

Supplier: New England Biolabs (NEB)

ProtoScript II First Strand cDNA Synthesis Kit - 150 rxns

ProtoScript II First Strand cDNA Synthesis Kit - 150 rxns

E6560S E6560L

CA102855-124EA 291 CAD

CA102855-124 CA102855-126

ProtoScript II First Strand cDNA Synthesis Kit, New England Biolabs

Nucleic Acid Reagents RT-PCR and cDNA Reagents

ProtoScript II First Strand cDNA Synthesis Kit features two optimized mixes, ProtoScript II Enzyme Mix and ProtoScript II Reaction Mix.

The enzyme mix combines ProtoScript II Reverse Transcriptase and Murine RNase Inhibitor, while the reaction mix contains dNTPs and an optimized buffer. ProtoScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48°C, providing higher specificity and higher yield of cDNA. The kit also provides two optimized primers for reverse transcription and nuclease-free water. An anchored Oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail. The optimized Random Primer Mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The first strand cDNA product generated is up to 10 kb.

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