Lugol's iodine solution stabilized for Gram staining, Sigma-Aldrich®

Supplier: MilliporeSigma

Stabilization: Stabilised with polyvinylpyrrolidon (PVP)
M-Clarity™ quality level = MQ300

1.00567.2500 1.00567.1000 1.00567.1000

CA1.00567.1000EA 113.04 CAD

EM1.00567.1000 CA1.00567.1000 EM1.00567.2500

Lugol's iodine solution stabilized for Gram staining, Sigma-Aldrich®

Lugol's iodine solution

Lugol’s solution stabilized with PVP is used in bacteriology, for example, for the staining of Gram bacteria.

It contains a complex of Iodine-Polyvinylpyrrolidon (PVP) and no free iodine, which stabilizes the solution and reduces the evaporation of iodine. During Gram-staining, the iodine from the Lugol’s solution and crystal violet form a stable complex that will not be released by decolorisation. Thus, Gram-positive bacteria will appear dark blue when analyzed microscopically, wheras Gram-negative bacteria can be counterstained e.g. orange with safranin. The Lugol’s solution is registered and quality controlled as IVD / CE product and can be used in diagnostics. 1 L of the Lugol's solution stabilized with PVP is capable of staining up to 500 slides.

Specification Test Results

Suitability for microscopy	passes test
gram-positve microorganisms	blue-violet
gram-negative microorganisms	pink to red

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