TBE 10X, Quality Biological

Supplier: Quality Biological

TBE 10X, Quality Biological

TBE 10X, Quality Biological

TBE 10X, Quality Biological

TBE 10X, Quality Biological

TBE 10X, Quality Biological

TBE 10X, Quality Biological

TBE 10X, Quality Biological

TBE 10X, Quality Biological

351-001-131CS 351-001-491 351-001-131

10128-686EA 430.66 CAD

10128-686 10128-682 10128-680

TBE 10X, Quality Biological

Buffers Electrophoresis Buffers Running Buffers

TBE (TRIS Borate EDTA) is often used in procedures involving nucleic acids, the most common being electrophoresis in molecular biology. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water.

Used both as a running buffer and as a gel preparation buffer
Filtered through a 0.22 µm membrane
DNase, RNase, and Protease tested

EDTA is a chelator of divalent cations, particularly of magnesium (Mg2+). As these ions are necessary co-factors for many enzymes, including contaminant nucleases, the role of the EDTA is to protect the nucleic acids against enzymatic degradation. But Mg2+ is also a co-factor for many useful DNA-modifying enzymes such as restriction enzymes and DNA polymerases.

TBE Buffer, 1X is used for DNA electrophoresis. TBE Buffer, 0.5X is used for electrophoresis onto DEAE cellulose membrane. TBE Buffer, 2X is used for polyacrylamide and agarose electrophoresis. TBE is also used for other nucleic acid electrophoresis procedures such as Northern and Southern blots.

Contents: Tris base, boric acid, disodium EDTA dihydrate, and molecular biology grade water.

Certifications: Manufactured under a cGMP compliant quality management system in an ISO 9001:2015 certified facility.

Caution: Research use only.

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