N,N,N',N'-Tetramethylethylenediamine (TEMED) ≥99%, liquid for electrophoresis
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
Synonyms:
1,2-Bis(dimethylamino)ethane
, N,N,N',N'-Tetramethylethylenediamine
, TMEDA
N,N,N',N'-Tetramethylethylenediamin is a tertiary amine base.
N,N,N',N'-Tetramethylethylenediamine is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules such as nucleic acids and proteins. TEMED has been used in the coating for soft polymers, such as polyacrylamide, in capillaries for investigation by atom force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED. It is also used used in the HPLC of small organic molecules.
Easily soluble in water (20 gm/mL-clear-cololess solution). Suggested use is 6-10 uL for 10 mL acrylamide gel solution (the less amount of TEMED used, the slower the polymerization)
Suitable for Polyacrylamide Gel Electrophoresis
|
Formula:
C₆H₁₆N₂ MW: 116.21 g/mol |
MDL Number:
MFCD00008335 CAS Number: 110-18-9 UN: 2372 ADR: 3,II |
|
Specification Test Results
| Synonyms | 1,2-Bis(dimethylamino)ethane; N,N,N′,N′-Tetramethylethylenediamine; TMEDA |
| Form | Liquid |
| Storage | Store at Room Temperature (15-30 °C), store under nitrogen. |
| Moisture Content | ≤2% |
| pH | 10 - 12 (0.5% aq soln) |
| Refractive Index | n20/D 1.4179 (Lit.) |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...